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Cell Rep. 2018 May 29;23(9):2819-2831.e5. doi: 10.1016/j.celrep.2018.04.114.

Similarities and Differences of Blood N-Glycoproteins in Five Solid Carcinomas at Localized Clinical Stage Analyzed by SWATH-MS.

Author information

1
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland. Electronic address: sajic@imsb.biol.ethz.ch.
2
Department of Pharmacology, Cancer Biology Institute, Yale University School of Medicine, West Haven, CT 06516, USA.
3
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland; PhD Program in Systems Biology, University of Zurich and ETH Zurich, Zurich, Switzerland.
4
UCL Cancer Institute, University College London, London, UK.
5
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland.
6
ProteoMediX AG, 8952 Schlieren, Switzerland.
7
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
8
Department of Biomedicine, University of Basel/University Hospital Basel, and Swiss Institute of Bioinformatics, Basel, Switzerland.
9
The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, 1825 Pressler, Houston, TX 77030, USA.
10
Department of Medicine, University of Washington, Seattle, WA 98195, USA.
11
Department of Clinical Sciences Lund, Surgery, Oncology and Pathology, Lund University, and Skåne University Hospital, Department of Surgery, Lund, Sweden.
12
Department of Clinical Sciences Lund, Oncology and Pathology, Lund University, and Skåne University Hospital, Department of Oncology, Lund, Sweden.
13
Department of Oncology and Hematology, Kantonsspital St. Gallen, St. Gallen, Switzerland; Department of Medical Oncology, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland.
14
Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland; Faculty of Science, University of Zurich, 8057 Zurich, Switzerland. Electronic address: aebersold@imsb.biol.ethz.ch.

Abstract

Cancer is mostly incurable when diagnosed at a metastatic stage, making its early detection via blood proteins of immense clinical interest. Proteomic changes in tumor tissue may lead to changes detectable in the protein composition of circulating blood plasma. Using a proteomic workflow combining N-glycosite enrichment and SWATH mass spectrometry, we generate a data resource of 284 blood samples derived from patients with different types of localized-stage carcinomas and from matched controls. We observe whether the changes in the patient's plasma are specific to a particular carcinoma or represent a generic signature of proteins modified uniformly in a common, systemic response to many cancers. A quantitative comparison of the resulting N-glycosite profiles discovers that proteins related to blood platelets are common to several cancers (e.g., THBS1), whereas others are highly cancer-type specific. Available proteomics data, including a SWATH library to study N-glycoproteins, will facilitate follow-up biomarker research into early cancer detection.

KEYWORDS:

SWATH-mass spectrometry; blood; carcinoma; glycoproteins; human clinical study; localized cancer; secreted

PMID:
29847809
DOI:
10.1016/j.celrep.2018.04.114
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