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Genes Immun. 2019 Jan;20(1):56-68. doi: 10.1038/s41435-017-0010-z. Epub 2018 Jan 24.

Choriodecidual leukocytes display a unique gene expression signature in spontaneous labor at term.

Author information

1
Departamento de Nutrición y Bioprogramación, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, MEX, Mexico.
2
Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Mexico City, MEX, Mexico.
3
Department of Obstetrics and Gynecology & Immunology and Microbiology, Wayne State University School of Medicine, Perinatal Research Initiative in Support of the Perinatology Research Branch, Detroit, MI, USA. nardhy.gomez-lopez@wayne.edu.
4
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Mexico City, MEX, Mexico.
5
Departamento de Genómica Computacional, Instituto Nacional de Medicina Genómica, Mexico City, MEX, Mexico.
6
Departamento de Micología y Virología, Instituto Nacional de Enfermedades Respiratorias, Mexico City, MEX, Mexico.
7
Departamento de Nutrición y Bioprogramación, Instituto Nacional de Perinatología Isidro Espinosa de los Reyes, Mexico City, MEX, Mexico. r.vega@inper.mx.

Abstract

Prior to and during the process of human labor, maternal circulating leukocytes infiltrate the maternal-fetal interface (choriodecidua) and become activated resembling choriodecidual leukocytes. Since, there is no evidence comparing maternal circulating and choriodecidual leukocytes, herein, we characterized their transcriptome and explored the biological processes enriched in choriodecidual leukocytes. From women undergoing spontaneous term labor we isolated circulating and choriodecidual leukocytes, performed microarray analysis (n = 5) and qRT-PCR validation (n = 9) and interaction network analysis with up-regulated genes. We found 270 genes up-regulated and only 17 genes down-regulated in choriodecidual leukocytes compared to maternal circulating leukocytes. The most up-regulated genes were CCL18, GPNMB, SEPP1, FN1, RNASE1, SPP1, C1QC, and PLTP. The biological processes enriched in choriodecidual leukocytes were cell migration and regulation of immune response, chemotaxis, and humoral immune responses. Our results show striking differences between the transcriptome of choriodecidual and maternal circulating leukocytes. Choriodecidual leukocytes are enriched in immune mediators implicated in the spontaneous process of labor at term.

PMID:
29362510
PMCID:
PMC6358585
DOI:
10.1038/s41435-017-0010-z
[Indexed for MEDLINE]
Free PMC Article

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