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Exp Ther Med. 2017 Dec;14(6):5464-5472. doi: 10.3892/etm.2017.5260. Epub 2017 Oct 3.

Identification of microRNAs in human circulating monocytes of postmenopausal osteoporotic Mexican-Mestizo women: A pilot study.

Author information

1
Genetics of Bone Metabolism Laboratory, National Institute of Genomic Medicine (INMEGEN), 14610 Mexico City, Mexico.
2
CONACYT, National Institute of Genomic Medicine (INMEGEN), 14610 Mexico City, Mexico.
3
Computational Genomics Consortium, National Institute of Genomic Medicine (INMEGEN), 14610 Mexico City, Mexico.
4
Oncogenomics Laboratory, National Institute of Genomic Medicine (INMEGEN), 14610 Mexico City, Mexico.
5
Center of Population Health Research, National Institute of Public Health (INSP), 62100 Cuernavaca-Morelos, Mexico.
6
Epidemiologic Research and Health Services Unit, Mexican Institute of Social Security (IMSS), 62000 Cuernavaca-Morelos, Mexico.

Abstract

MicroRNAs (miRNAs or miRs) are a class of short non-coding RNAs that serve an important regulatory role in living organisms. These molecules are associated with multiple biological processes and are potential biomarkers in multiple diseases. The present study aimed to further identify miRNAs that are differentially expressed in circulating monocytes (CMCs) from postmenopausal Mexican-Mestizo women. Microarray analyses of monocytes using Affymetrix miRNA 4.0 and Human Genome U133 Plus 2.0 arrays were performed in 6 normal and 6 osteoporotic women, followed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validation. The overexpression of miR-1270, miR-548×-3p and miR-8084 were detected in the osteoporosis compared with the normal group according to the microarray analysis; miR-1270, a miRNA with several target genes associated with bone remodeling, was validated by RT-qPCR. Bioinformatics analysis identified that interferon regulatory factor 8 (IRF8) is the most likely target gene of miR-1270, which is associated with osteoclastogenesis. Furthermore, the findings of the present study demonstrate that an upregulation of miR-1270 may reduce the gene expression of IRF8 in CMCs (osteoclast precursors), implicating its potential role in leading to low bone mineral density and contributing to osteoporosis development in postmenopausal women.

KEYWORDS:

expression; microRNAs; monocytes; osteoclasts; osteoporosis

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