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Eur J Cell Biol. 2017 Dec;96(8):767-773. doi: 10.1016/j.ejcb.2017.10.002. Epub 2017 Oct 14.

The fate of multilamellar bodies produced and secreted by Dictyostelium discoideum amoebae.

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1
Institut de Biologie Intégrative et des Systèmes, Pavillon Charles-Eugène-Marchand, Université Laval, Quebec City, QC, Canada; Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Hôpital Laval, Quebec City, QC, Canada; Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Université Laval, Quebec City, QC, Canada.
2
Institut de Biologie Intégrative et des Systèmes, Pavillon Charles-Eugène-Marchand, Université Laval, Quebec City, QC, Canada; Centre de recherche de l'Institut universitaire de cardiologie et de pneumologie de Québec, Hôpital Laval, Quebec City, QC, Canada; Département de biochimie, de microbiologie et de bio-informatique, Faculté des sciences et de génie, Université Laval, Quebec City, QC, Canada. Electronic address: steve.charette@bcm.ulaval.ca.

Abstract

The amoeba Dictyostelium discoideum produces and secretes multilamellar bodies (MLBs) mainly composed of amoebal membranes upon digestion of bacteria. After their secretion, the fate of these MLBs remains unknown. The aim of this study was to determine if protozoa can internalize and digest secreted D. discoideum MLBs. Our results showed that MLBs were ingested by naive axenic D. discoideum cells (i. e. cells not exposed to bacteria and consequently not producing MLBs). Only a small fraction of the ingested MLBs were found in cells' post-lysosomes compared to undigestible beads suggesting that naive amoebae digest them. D. discoideum MLBs were also ingested by the ciliates Tetrahymena pyriformis and Tetrahymena thermophila. MLBs internalized by the ciliates were compacted into pellets and expelled in the extracellular medium without obvious signs of degradation. The results of this study provide new insights on the biological function of MLBs and, considering that MLBs are also involved in bacteria packaging, suggest additional layers of complexity in microbial interactions.

KEYWORDS:

Dictyostelium discoideum; Exocytosis; Fecal pellets; Multilamellar bodies; Phagocytosis; Tetrahymena

PMID:
29107380
DOI:
10.1016/j.ejcb.2017.10.002
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