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Genetics. 2017 Dec;207(4):1371-1386. doi: 10.1534/genetics.117.300322. Epub 2017 Oct 4.

Long-Chain Polyprenols Promote Spore Wall Formation in Saccharomyces cerevisiae.

Author information

1
Department of Biochemistry and Cell Biology, Stony Brook University, New York 11794-5215.
2
Department of Pharmacology, Yale School of Medicine, New Haven, Connecticut 06520-8066.
3
Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina 27710.
4
Department of Biochemistry and Cell Biology, Stony Brook University, New York 11794-5215 aaron.neiman@stonybrook.edu.

Abstract

Dolichols are isoprenoid lipids of varying length that act as sugar carriers in glycosylation reactions in the endoplasmic reticulum. In Saccharomyces cerevisiae, there are two cis-prenyltransferases that synthesize polyprenol-an essential precursor to dolichol. These enzymes are heterodimers composed of Nus1 and either Rer2 or Srt1. Rer2-Nus1 and Srt1-Nus1 can both generate dolichol in vegetative cells, but srt1∆ cells grow normally while rer2∆ grows very slowly, indicating that Rer2-Nus1 is the primary enzyme used in mitotically dividing cells. In contrast, SRT1 performs an important function in sporulating cells, where the haploid genomes created by meiosis are packaged into spores. The spore wall is a multilaminar structure and SRT1 is required for the generation of the outer chitosan and dityrosine layers of the spore wall. Srt1 specifically localizes to lipid droplets associated with spore walls, and, during sporulation there is an SRT1-dependent increase in long-chain polyprenols and dolichols in these lipid droplets. Synthesis of chitin by Chs3, the chitin synthase responsible for chitosan layer formation, is dependent on the cis-prenyltransferase activity of Srt1, indicating that polyprenols are necessary to coordinate assembly of the spore wall layers. This work shows that a developmentally regulated cis-prenyltransferase can produce polyprenols that function in cellular processes besides protein glycosylation.

KEYWORDS:

Chs3; Srt1; chitosan; cis-prenyltransferase; dolichol; lipid droplet; sporulation; yeast

PMID:
28978675
PMCID:
PMC5714454
DOI:
10.1534/genetics.117.300322
[Indexed for MEDLINE]
Free PMC Article

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