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Biol Open. 2017 Nov 15;6(11):1629-1643. doi: 10.1242/bio.028233.

An evolutionarily conserved phosphatidate phosphatase maintains lipid droplet number and endoplasmic reticulum morphology but not nuclear morphology.

Author information

1
School of Biological Sciences, National Institute of Science Education and Research (NISER)-Bhubaneswar, HBNI, P.O. Jatni, Khurda 752050, Odisha, India.
2
School of Biological Sciences, National Institute of Science Education and Research (NISER)-Bhubaneswar, HBNI, P.O. Jatni, Khurda 752050, Odisha, India arahaman@niser.ac.in.

Abstract

Phosphatidic acid phosphatases are involved in the biosynthesis of phospholipids and triacylglycerol, and also act as transcriptional regulators. Studies to ascertain their role in lipid metabolism and membrane biogenesis are restricted to Opisthokonta and Archaeplastida. Here, we report the role of phosphatidate phosphatase (PAH) in Tetrahymena thermophila, belonging to the Alveolata clade. We identified two PAH homologs in Tetrahymena, TtPAH1 and TtPAH2 Loss of function of TtPAH1 results in reduced lipid droplet number and an increase in endoplasmic reticulum (ER) content. It also results in more ER sheet structure as compared to wild-type Tetrahymena Surprisingly, we did not observe a visible defect in the nuclear morphology of the ΔTtpah1 mutant. TtPAH1 rescued all known defects in the yeast pah1Δ strain and is conserved functionally between Tetrahymena and yeast. The homologous gene derived from Trypanosoma also rescued the defects of the yeast pah1Δ strain. Our results indicate that PAH, previously known to be conserved among Opisthokonts, is also present in a set of distant lineages. Thus, a phosphatase cascade is evolutionarily conserved and is functionally interchangeable across eukaryotic lineages.

KEYWORDS:

Endoplasmic reticulum; Lipid droplet; Lipin; Nuclear membrane expansion; Phosphatidic acid hydrolase; Tetrahymena thermophila

Conflict of interest statement

Competing interestsThe authors declare no competing or financial interests.

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