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Biosens Bioelectron. 2018 Feb 15;100:396-403. doi: 10.1016/j.bios.2017.09.031. Epub 2017 Sep 20.

Development of a bifunctional nanobiosensor for screening and detection of chemokine ligand in colorectal cancer cell line.

Author information

1
Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea.
2
Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
3
Department of Internal Medicine, Yale Cancer Center, Yale School of Medicine, 333 Cedar Street, New Haven, CT 06520, USA.
4
Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan 609-735, South Korea. Electronic address: ybshim@pusan.ac.kr.

Abstract

Highly sensitive detection of chemokines in various biological matrices and its interaction with a natural receptor molecule has tremendous importance in cell signaling, medical diagnostics, and therapeutics. In this direction, we have designed the first bifunctional nanobiosensor for chemokine screening and detection in a single experimental setting. The sensor probe was fabricated by immobilizing CXCR2 on the gold nanoparticles (AuNPs) deposited 2,2':5',2''-terthiophene-3' (p-benzoic acid) (TBA) nanocomposite film. The interaction between CXCR2 and chemokines was studied using electrochemical impedance spectroscopy (EIS) and voltammetry. CXCL5 among three ligands showed the strongest affinity to CXCR2, which was further utilized to develop an amperometric CXCL5 biosensor. Analytical parameters, such as CXCR2 receptor concentration, temperature, pH, and incubation time were optimized to obtain the high sensitivity. A dynamic range for CXCL5 detection was obtained between 0.1 and 10ng/mL with the detection limit of 0.078 ± 0.004ng/mL (RSD < 4.7%). The proposed biosensor was successfully applied to detect CXCL5 in clinically relevant concentrations in human serum and colorectal cancer cells samples with high sensitivity and selectivity. Interference effect and the stability of the developed biosensor were also evaluated. Method verification was performed by comparing the results using commercially available ELISA kit for CXCL5 detection.

KEYWORDS:

Bifunctional nanobiosensor; Cancer, Colorectal cancer cells; Chemokine; Human Health; Human serum; In vitro diagnosis

PMID:
28954256
DOI:
10.1016/j.bios.2017.09.031
[Indexed for MEDLINE]

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