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Methods Mol Biol. 2017;1657:285-289. doi: 10.1007/978-1-4939-7240-1_22.

Determining Diguanylate Cyclase Activity (Radioactive Assay).

Author information

1
Department of Medicine, Yale University, 333 Cedar St., New Haven, CT, 06520-8022, USA. barbara.kazmierczak@yale.edu.
2
Department of Microbial Pathogenesis, Yale University, 333 Cedar St., New Haven, CT, 06520-8022, USA. barbara.kazmierczak@yale.edu.

Abstract

Bioinformatics approaches can identify sequence motifs associated with diguanylate cyclases (DGCs), but experimental demonstration of DGC enzymatic activity is often desired. This protocol describes a sensitive radioactive assay for DGC activity in which substrate and product are quickly and easily separated by thin-layer chromatography.

KEYWORDS:

Cyclic-di-GMP; Diguanylate cyclase; Thin-layer chromatography

PMID:
28889302
DOI:
10.1007/978-1-4939-7240-1_22
[Indexed for MEDLINE]

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