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Mol Biochem Parasitol. 2017 Sep;216:52-55. doi: 10.1016/j.molbiopara.2017.07.002. Epub 2017 Jul 14.

Metacyclic VSG expression site promoters are recognized by the same general transcription factor that is required for RNA polymerase I transcription of bloodstream expression sites.

Author information

1
Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06536, USA. Electronic address: nikolay.kolev@yale.edu.
2
Department of Genetics and Genome Sciences, University of Connecticut Health Center, Farmington, CT 06030, USA.
3
Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06536, USA.

Abstract

Infectious metacyclic Trypanosoma brucei cells develop in the salivary glands of tsetse flies. A critical aspect of the developmental program leading to acquisition of infectivity is the synthesis of a variant surface glycoprotein (VSG) coat. Metacyclic VSG genes are transcribed from a set of specialized VSG expression sites (ESs) that differ from bloodstream VSG ESs by being monocistronic, being significantly shorter, lacking long stretches of 70-bp repeats, and having distinct promoter sequences. Both metacyclic and bloodstream VSG ESs are transcribed by the multifunctional T. brucei RNA polymerase I (Pol I), however the factor that recognizes the divergent metacyclic VSG ES promoters and recruits Pol I during the development to infectious cells remains unknown. We used an in vitro assay to show that the promoters for both metacyclic and bloodstream VSG ESs are recognized by the same class I transcription factor A (CITFA). This general Pol I transcription initiation factor was previously shown to be essential for the transcription of bloodstream VSG genes, procyclin genes and rRNA genes, and was demonstrated to have distinct binding affinities for these three types of promoters. We now show that differences in the sequence of individual metacyclic VSG ESs promoters determine different affinities for CITFA.

KEYWORDS:

CITFA; Metacyclics; Pol I; Transcription; Trypanosoma brucei; VSG

PMID:
28716719
PMCID:
PMC5582990
DOI:
10.1016/j.molbiopara.2017.07.002
[Indexed for MEDLINE]
Free PMC Article

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