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J Mol Biol. 2017 Oct 27;429(21):3301-3318. doi: 10.1016/j.jmb.2017.04.015. Epub 2017 Apr 26.

The Canonical Poly (A) Polymerase PAP1 Polyadenylates Non-Coding RNAs and Is Essential for snoRNA Biogenesis in Trypanosoma brucei.

Author information

1
The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University, Ramat-Gan 5290002, Israel.
2
Department of Epidemiology and Microbial Diseases, Yale School of Public Health, New Haven, CT 06536, USA.
3
Department of Internal Medicine, Yale University Medical School, 295 Congress Avenue, New Haven, CT 06536-0812, USA; Cell Biology, Yale University Medical School, 295 Congress Avenue, New Haven, CT 06536-0812, USA.
4
The Mina and Everard Goodman Faculty of Life Sciences and Advanced Materials and Nanotechnology Institute, Bar-Ilan University, Ramat-Gan 5290002, Israel. Electronic address: shulamit.michaeli@biu.ac.il.

Abstract

The parasite Trypanosoma brucei is the causative agent of African sleeping sickness and is known for its unique RNA processing mechanisms that are common to all the kinetoplastidea including Leishmania and Trypanosoma cruzi. Trypanosomes possess two canonical RNA poly (A) polymerases (PAPs) termed PAP1 and PAP2. PAP1 is encoded by one of the only two genes harboring cis-spliced introns in this organism, and its function is currently unknown. In trypanosomes, all mRNAs, and non-coding RNAs such as small nucleolar RNAs (snoRNAs) and long non-coding RNAs (lncRNAs), undergo trans-splicing and polyadenylation. Here, we show that the function of PAP1, which is located in the nucleus, is to polyadenylate non-coding RNAs, which undergo trans-splicing and polyadenylation. Major substrates of PAP1 are the snoRNAs and lncRNAs. Under the silencing of either PAP1 or PAP2, the level of snoRNAs is reduced. The dual polyadenylation of snoRNA intermediates is carried out by both PAP2 and PAP1 and requires the factors essential for the polyadenylation of mRNAs. The dual polyadenylation of the precursor snoRNAs by PAPs may function to recruit the machinery essential for snoRNA processing.

KEYWORDS:

Trypanosomes; canonical polyadenylation; non-coding RNAs; poly (A) polymerase; snoRNA maturation

PMID:
28456523
DOI:
10.1016/j.jmb.2017.04.015
[Indexed for MEDLINE]

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