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Placenta. 2017 Apr;52:17-20. doi: 10.1016/j.placenta.2017.02.010. Epub 2017 Feb 13.

Cytogenetic features of human trophoblast cell lines SWAN-71 and 3A-subE.

Author information

1
Department of Obstetrics and Gynecology, Indiana University School of Medicine, 550 N. University Ave, UH 2440, Indianapolis, IN 46202, USA. Electronic address: jireiter@iu.edu.
2
Department of Medical & Molecular Genetics, Indiana University School of Medicine, 975 W Walnut St, Indianapolis, IN 46202, USA.
3
Department of Obstetrics and Gynecology, Indiana University School of Medicine, 550 N. University Ave, UH 2440, Indianapolis, IN 46202, USA.
4
Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, 333 Cedar St, LSOG 312, New Haven, CT 06510, USA.

Abstract

Immortalization of primary cells with telomerase is thought to maintain normal phenotypic properties and avoid chromosomal abnormalities and other cancer-associated changes that occur following simian virus 40 tumor antigen (SV40 Tag) induced immortalization. However, we report that the human telomerase reverse transcriptase (hTERT)-immortalized SWAN-71 trophoblast cell line has a near pentaploid 103∼119,XXXX[cp20] karyotype. Additionally, DNA typing analysis indicated that SWAN-71 cells have acquired microsatellite instability. In comparison, the post-crisis SV40-transformed trophoblast cell line 3A-subE was hypertriploid 69∼81,XX[cp20]. Both cell lines contained multiple specific clonal rearrangements. These findings emphasize the need to monitor for genetic instability in hTERT-immortalized cells.

KEYWORDS:

Cellular immortalization; Karyotype; Polyploidy; Short tandem repeat profile; Telomerase; Trophoblast cell lines

PMID:
28454693
DOI:
10.1016/j.placenta.2017.02.010
[Indexed for MEDLINE]
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