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Cell. 2017 Apr 20;169(3):547-558.e15. doi: 10.1016/j.cell.2017.03.045.

Engineered Regulatory Systems Modulate Gene Expression of Human Commensals in the Gut.

Author information

1
Department of Microbial Pathogenesis and Microbial Sciences Institute, Yale University School of Medicine, New Haven, CT 06536-0812, USA.
2
Department of Microbial Pathogenesis and Microbial Sciences Institute, Yale University School of Medicine, New Haven, CT 06536-0812, USA. Electronic address: andrew.goodman@yale.edu.

Abstract

The gut microbiota is implicated in numerous aspects of health and disease, but dissecting these connections is challenging because genetic tools for gut anaerobes are limited. Inducible promoters are particularly valuable tools because these platforms allow real-time analysis of the contribution of microbiome gene products to community assembly, host physiology, and disease. We developed a panel of tunable expression platforms for the prominent genus Bacteroides in which gene expression is controlled by a synthetic inducer. In the absence of inducer, promoter activity is fully repressed; addition of inducer rapidly increases gene expression by four to five orders of magnitude. Because the inducer is absent in mice and their diets, Bacteroides gene expression inside the gut can be modulated by providing the inducer in drinking water. We use this system to measure the dynamic relationship between commensal sialidase activity and liberation of mucosal sialic acid, a receptor and nutrient for pathogens. VIDEO ABSTRACT.

KEYWORDS:

Bacteroides; anhydrotetracycline; gene regulation; gut; inducible promoter; microbiome; sialic acid; synthetic biology

PMID:
28431252
PMCID:
PMC5532740
DOI:
10.1016/j.cell.2017.03.045
[Indexed for MEDLINE]
Free PMC Article

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