Format

Send to

Choose Destination
See comment in PubMed Commons below
Clin Breast Cancer. 2017 Jan 27. pii: S1526-8209(16)30479-7. doi: 10.1016/j.clbc.2017.01.007. [Epub ahead of print]

Expression Profiling of Clinical Specimens Supports the Existence of Neural Progenitor-Like Stem Cells in Basal Breast Cancers.

Author information

1
Department of Surgery, Section of Otolaryngology, Yale School of Medicine, New Haven, CT.
2
Department of Cancer Biology, Vanderbilt University School of Medicine, Nashville, TN.
3
Department of Surgery, Section of Otolaryngology, Yale School of Medicine, New Haven, CT; Head and Neck Disease Center, Smilow Cancer Hospital, New Haven, CT; Molecular Virology Program, Yale Cancer Center, New Haven, CT.
4
Department of Surgery, Section of Otolaryngology, Yale School of Medicine, New Haven, CT. Electronic address: sergey.ivanov@yale.edu.

Abstract

BACKGROUND:

We previously characterized in salivary adenoid cystic carcinoma (ACC) a novel population of cancer stem cells (CSCs) marked by coexpression of 2 stemness genes, sex-determining region Y (SRY)-related HMG box-containing factor 10 (SOX10) and CD133. We also reported that in ACC and basal-like breast carcinoma (BBC), a triple-negative breast cancer subtype, expression of SOX10 similarly demarcates a highly conserved gene signature enriched with neural stem cell genes. On the basis of these findings, we hypothesized that BBC might be likewise driven by SOX10-positive (SOX10+)/CD133+ cells with neural stem cell properties.

MATERIALS AND METHODS:

To validate our hypothesis on clinical data, we used a novel approach to meta-analysis that merges gene expression data from independent breast cancer studies and ranks genes according to statistical significance of their coexpression with the gene of interest. Genes that showed strong association with CD133/PROM1 as well as SOX10 were validated across different platforms and data sets and analyzed for enrichment with genes involved in neurogenesis.

RESULTS:

We identified in clinical breast cancer data sets a highly conserved SOX10/PROM1 gene signature that contains neural stem cell markers common for Schwann cells, ACC, BBC, and melanoma. Identification of tripartite motif-containing 2 (TRIM2), TRIM29, MPZL2, potassium calcium-activated channel subfamily N member 4 (KCNN4), and V-set domain containing T cell activation inhibitor 1 (VTCN1)/B7 homolog 4 (B7H4) within this signature provides insight into molecular mechanisms of CSC maintenance.

CONCLUSION:

Our results suggest that BBC is driven by SOX10+/CD133+ cells that express neural stem cell-specific markers and share molecular similarities with CSCs of neural crest origin. Our study provides clinically relevant information on possible drivers of these cells that might facilitate development of CSC-targeting therapies against this cancer distinguished with poor prognosis and resistance to conventional therapies.

KEYWORDS:

Adenoid cystic salivary carcinoma; Basal-like breast carcinoma; CD133; Cancer stem cells

PMID:
28216417
DOI:
10.1016/j.clbc.2017.01.007
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center