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Virology. 2017 Apr;504:152-167. doi: 10.1016/j.virol.2017.01.022. Epub 2017 Feb 11.

Genetic characterization, molecular epidemiology, and phylogenetic relationships of insect-specific viruses in the taxon Negevirus.

Author information

1
Center for Technological Innovation, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
2
Programa de Estudio y Control de Enfermedades Tropicales - PECET - SIU-Sede de Investigación Universitaria - Universidad de Antioquia, Medellín, Colombia; Grupo de Investigación en Sistemática Molecular-GSM, Facultad de Ciencias,Ciencias, Universidad Nacional de Colombia, sede Medellín, Medellín, Colombia.
3
Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, United States; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, United States; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
4
Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil.
5
Vale S.A. - Projeto Ferro Carajás S11D, Canaã dos Carajás, Para, Brazil.
6
Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT 06520, United States.
7
School of Public Health, University of Washington, Seattle, WA 98195, United States.
8
Grupo de Investigación en Sistemática Molecular-GSM, Facultad de Ciencias,Ciencias, Universidad Nacional de Colombia, sede Medellín, Medellín, Colombia.
9
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX 77555-0645, United States.
10
Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06520, United States.
11
Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, United States; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, United States; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil. Electronic address: nivasila@utmb.edu.
12
Department of Pathology and Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, United States; Center for Tropical Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, United States; Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, Para, Brazil. Electronic address: rtesh@utmb.edu.

Abstract

The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.

KEYWORDS:

Insect-specific viruses; Negevirus; Nelorpivirus genus; Sandewavirus genus; Virus diversity

PMID:
28193550
PMCID:
PMC5394984
DOI:
10.1016/j.virol.2017.01.022
[Indexed for MEDLINE]
Free PMC Article

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