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Am J Reprod Immunol. 2017 Apr;77(4). doi: 10.1111/aji.12637. Epub 2017 Jan 31.

Time course analysis of RNA quality in placenta preserved by RNAlater or flash freezing.

Author information

1
Department of Obstetrics and Gynecology and Reproductive Sciences, Yale School of Medicine, New Haven, CT, USA.

Abstract

PROBLEM:

Preservation of biospecimen quality is critical to accurately and reliably assessing genes and proteins. We evaluated the effect of preparation method and storage duration on RNA quality in placenta and decidua.

METHOD OF STUDY:

Aliquots of nine placentas and decidua were placed in RNAlater® (RL) or flash frozen (FF) within 30 minutes of delivery. RNA was extracted immediately (baseline) and from matched samples stored at -80°C for 1 and 8-10 months. RNA Integrity Number (RIN) and housekeeping gene expression were quantified.

RESULTS:

At both time points, RL placenta had RIN and housekeeping gene Ct values similar to baseline. However, FF placenta had significantly lower RIN and higher Ct values at 1 and 8-10 months. In RL and FF decidua, RIN was unchanged from baseline.

CONCLUSION:

We found RNAlater more effectively and consistently preserved placenta, compared to flash freezing. However, for decidua, which is less dense than placenta, both modes yielded comparable RNA integrity over time.

KEYWORDS:

RNA Integrity Number; decidua; housekeeping gene

PMID:
28138995
PMCID:
PMC5397349
DOI:
10.1111/aji.12637
[Indexed for MEDLINE]
Free PMC Article

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