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Elife. 2017 Jan 9;6. pii: e18855. doi: 10.7554/eLife.18855.

Metabolite exchange between microbiome members produces compounds that influence Drosophila behavior.

Author information

1
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, United States.
2
Department of Chemistry, Yale University, New Haven, United States.
3
Department of Microbiology, University of Georgia, Athens, United States.
4
Department of Molecular and Cell Biology, University of Connecticut, Storrs, United States.
5
Institute for Systems Genomics, University of Connecticut, Storrs, United States.

Abstract

Animals host multi-species microbial communities (microbiomes) whose properties may result from inter-species interactions; however, current understanding of host-microbiome interactions derives mostly from studies in which elucidation of microbe-microbe interactions is difficult. In exploring how Drosophila melanogaster acquires its microbiome, we found that a microbial community influences Drosophila olfactory and egg-laying behaviors differently than individual members. Drosophila prefers a Saccharomyces-Acetobacter co-culture to the same microorganisms grown individually and then mixed, a response mainly due to the conserved olfactory receptor, Or42b. Acetobacter metabolism of Saccharomyces-derived ethanol was necessary, and acetate and its metabolic derivatives were sufficient, for co-culture preference. Preference correlated with three emergent co-culture properties: ethanol catabolism, a distinct volatile profile, and yeast population decline. Egg-laying preference provided a context-dependent fitness benefit to larvae. We describe a molecular mechanism by which a microbial community affects animal behavior. Our results support a model whereby emergent metabolites signal a beneficial multispecies microbiome.

KEYWORDS:

D. melanogaster; S. cerevisiae; ecology; host-microbe interactions; infectious disease; metabolism; microbe-microbe interactions; microbiology; microbiota; olfaction

PMID:
28068220
PMCID:
PMC5222558
DOI:
10.7554/eLife.18855
[Indexed for MEDLINE]
Free PMC Article

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