Format

Send to

Choose Destination
See comment in PubMed Commons below
Gene. 2017 Mar 20;605:99-107. doi: 10.1016/j.gene.2016.12.030. Epub 2016 Dec 31.

MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A.

Author information

1
Department of Pharmaceutical Science, Shenzhen People's Hospital, Second Clinical College, Jinan University, Shenzhen 518020, China.
2
Department of Pathology, Yale University School of Medicine, New Haven 06520, USA; Shanghai Tenth People's Hospital, Medical School, Tongji University, Shanghai 200072, China. Electronic address: te.liu@yale.edu.
3
Laboratoire PROTEE, Bâtiment R, Université du Sud Toulon-Var, 83957 La Garde Cedex, France.
4
Department of Medical Oncology, Shanghai Changzheng Hospital, Affiliated to The Second Military Medical University, Shanghai 200070, China.
5
Department of Pharmaceutical Science, Shenzhen People's Hospital, Second Clinical College, Jinan University, Shenzhen 518020, China. Electronic address: yht2113@126.com.
6
Gongli Hospital Affiliated to the Second Military Medicical University in Pudong New Area of Shanghai City, Shanghai 200135, China. Electronic address: 406526258@qq.com.

Abstract

The cluster of microRNAs (miRNAs) in the DLK1-DIO3 genomic imprinted region contains several miRNAs that have a significant regulatory role in tumor proliferation and invasion. One of these miRNAs is miR-134-3p, and its expression changes significantly in human ovarian cancer stem cells (OCSCs) and in CD44-/CD133- ovarian cancer. The results of a luciferase assay showed that miR-134-3p silenced RAB27A by binding to the 3'-UTR of RAB27A mRNA. Overexpression of miR-134-3p in human OCSCs can not only inhibit the expression of RAB27A but also can effectively downregulate the expression of some tumor proliferation and invasion genes. Overexpression of miR-134-3p can not only inhibit the in vitro proliferation and cell cycle progression of human OCSCs but also can decrease the tumorigenicity in nude mice.

KEYWORDS:

DLK1-DIO3 microRNA cluster; MicroRNA-134-3p (miR-134-3p); Ovarian cancer stem cells (OCSCs); RAB27A

PMID:
28043921
DOI:
10.1016/j.gene.2016.12.030
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center