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VAD J. 2016;2(1). doi: 10.13023/VAD.2016.12. Epub 2016 Jun 3.

A Protocol for Collecting Human Cardiac Tissue for Research.

Author information

1
Department of Physiology, University of Kentucky, Lexington, KY.
2
Department of Pediatrics, Institute for Stem Cell & Regenerative Medicine, University of Washington, Seattle, WA.
3
Department of Biomedical Engineering, Yale University, New Haven, CT.
4
Department of Physiology, Wayne State University, Detroit, MI.
5
Markey Cancer Center, University of Kentucky, Lexington, KY.
6
Gill Heart Institute, University of Kentucky, Lexington, KY.
7
University of Toledo Medical Center, Toledo, OH.
8
University of Alabama Birmingham School of Medicine, Birmingham, AL.
9
Gill Heart Institute, University of Kentucky, Lexington, KY; Division of Cardiovascular Medicine, University of Kentucky, Lexington, KY.
10
Department of Physiology, University of Kentucky, Lexington, KY; Division of Cardiovascular Medicine, University of Kentucky, Lexington, KY.

Abstract

This manuscript describes a protocol at the University of Kentucky that allows a translational research team to collect human myocardium that can be used for biological research. We have gained a great deal of practical experience since we started this protocol in 2008, and we hope that other groups might be able to learn from our endeavors. To date, we have procured ~4000 samples from ~230 patients. The tissue that we collect comes from organ donors and from patients who are receiving a heart transplant or a ventricular assist device because they have heart failure. We begin our manuscript by describing the importance of human samples in cardiac research. Subsequently, we describe the process for obtaining consent from patients, the cost of running the protocol, and some of the issues and practical difficulties that we have encountered. We conclude with some suggestions for other researchers who may be considering starting a similar protocol.

KEYWORDS:

Cardiac; Heart failure; Human; Ventricular assist device

PMID:
28042604
PMCID:
PMC5199025
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