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Traffic. 2017 Feb;18(2):134-144. doi: 10.1111/tra.12462. Epub 2017 Jan 16.

Distinct complexes of yeast Snx4 family SNX-BARs mediate retrograde trafficking of Snc1 and Atg27.

Author information

1
Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut.
2
Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, North Carolina.

Abstract

The yeast SNX4 sub-family of sorting nexin containing a Bin-Amphiphysin-Rvs domain (SNX-BAR) proteins, Snx4/Atg24, Snx41 and Atg20/Snx42, are required for endocytic recycling and selective autophagy. Here, we show that Snx4 forms 2 functionally distinct heterodimers: Snx4-Atg20 and Snx4-Snx41. Each heterodimer coats an endosome-derived tubule that mediates retrograde sorting of distinct cargo; the v-SNARE, Snc1, is a cargo of the Snx4-Atg20 pathway, and Snx4-Snx41 mediates retrograde sorting of Atg27, an integral membrane protein implicated in selective autophagy. Live cell imaging of individual endosomes shows that Snx4 and the Vps5-Vps17 retromer SNX-BAR heterodimer operate concurrently on a maturing endosome. Consistent with this, the yeast dynamin family protein, Vps1, which was previously shown to promote fission of retromer-coated tubules, promotes fission of Snx4-Atg20 coated tubules. The results indicate that the yeast SNX-BAR proteins coat 3 distinct types of endosome-derived carriers that mediate endosome-to-Golgi retrograde trafficking.

KEYWORDS:

SNX-BAR ; Atg27; Vps1; autophagy; endosome; retromer

PMID:
28026081
PMCID:
PMC5262529
DOI:
10.1111/tra.12462
[Indexed for MEDLINE]
Free PMC Article

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