Format

Send to

Choose Destination
Methods Mol Biol. 2017;1507:261-268.

Large-Scale RNA Interference Screening to Identify Transcriptional Regulators of a Tumor Suppressor Gene.

Author information

1
Department of Pathology, Yale University School of Medicine, 310 Cedar Street, 208023, New Haven, CT, 06518, USA.
2
Department of Pathology, Yale University School of Medicine, 310 Cedar Street, 208023, New Haven, CT, 06518, USA. Narendra.Wajapeyee@yale.edu.

Abstract

RNA interference (RNAi) is a powerful research tool that can be used to silence the expression of a specific gene. In the past several years, RNAi has provided the opportunity to identify factors and pathways involved in complex biological processes by performing unbiased loss-of-function screens on a genome-wide scale. Here we describe a genome-wide RNAi screening strategy to identify factors that regulates epigenetic silencing of a specific tumor suppressor gene, using RASSF1A as an example. The approach we describe is a general RNAi screening strategy that can be applied to identify other factors that drive and/or maintain epigenetic modifications on specific genes, including cancer-related genes.

KEYWORDS:

Epigenetic silencing; Positive-selection screen; RNA interference; shRNA; siRNA

PMID:
27832546
PMCID:
PMC5340288
DOI:
10.1007/978-1-4939-6518-2_19
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Springer Icon for PubMed Central
Loading ...
Support Center