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Sci Rep. 2016 Nov 7;6:36791. doi: 10.1038/srep36791.

Serum neurofilament light protein predicts clinical outcome in traumatic brain injury.

Author information

1
Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, Sahlgrenska Academy at University of Gothenburg, Sahlgrenska University Hospital, Mölndal, SE-43180 Mölndal, Sweden.
2
UmanDiagnostics, Umeå, Sweden.
3
Division of Medical Sciences, Department of Health Sciences, Luleå University of Technology, SE 971 87 Luleå, Sweden.
4
Clinical Memory Research Unit, Lund University, Malmö, Sweden.
5
Department of NVS, Karolinska Institute, Center for Alzheimer Research, Stockholm, Sweden.
6
Department of Anaesthesiology and Intensive Care, Institute of Clinical Sciences, Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden.
7
Department of Molecular Neuroscience, UCL Institute of Neurology, London WC1N1PJ, UK.

Abstract

Axonal white matter injury is believed to be a major determinant of adverse outcomes following traumatic brain injury (TBI). We hypothesized that measurement of neurofilament light protein (NF-L), a protein found in long white-matter axons, in blood samples, may serve as a suitable biomarker for neuronal damage in TBI patients. To test our hypotheses, we designed a study in two parts: i) we developed an immunoassay based on Single molecule array technology for quantification of NF-L in blood, and ii) in a proof-of-concept study, we tested our newly developed method on serial serum samples from severe TBI (sTBI) patients (n = 72) and controls (n = 35). We also compared the diagnostic and prognostic utility of NF-L with the established blood biomarker S100B. NF-L levels were markedly increased in sTBI patients compared with controls. NF-L at admission yielded an AUC of 0.99 to detect TBI versus controls (AUC 0.96 for S100B), and increased to 1.00 at day 12 (0.65 for S100B). Importantly, initial NF-L levels predicted poor 12-month clinical outcome. In contrast, S100B was not related to outcome. Taken together, our data suggests that measurement of serum NF-L may be useful to assess the severity of neuronal injury following sTBI.

PMID:
27819296
PMCID:
PMC5098187
DOI:
10.1038/srep36791
[Indexed for MEDLINE]
Free PMC Article

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