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Am J Trop Med Hyg. 2017 Jan 11;96(1):100-109. doi: 10.4269/ajtmh.16-0403. Epub 2016 Oct 31.

Almendravirus: A Proposed New Genus of Rhabdoviruses Isolated from Mosquitoes in Tropical Regions of the Americas.

Author information

1
Programa de Estudio y Control de Enfermedades Tropicales (PECET), Sede de Investigacion Universitaria (SIU), Universidad de Antioquia, Medellin, Colombia.
2
Grupo de Investigacion en Sistematica Molecular (GSM), Facultad de Ciencias, Universidad Nacional de Colombia, Medellin, Colombia.
3
Griffin Arbovirus Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York.
4
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, Center for Tropical Diseases, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas.
5
Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut.
6
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas.
7
Yale School of Public Health, New Haven, Connecticut.
8
Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, Center for Tropical Diseases, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas. peter.walker@uq.edu.au nivasila@utmb.edu.
9
CSIRO Health and Biosecurity, Australian Animal Health Laboratory, Geelong, Australia. peter.walker@uq.edu.au nivasila@utmb.edu.
10
School of Biological Sciences, University of Queensland, St Lucia, Australia.

Abstract

The Rhabdoviridae is a diverse family of negative-sense single-stranded RNA viruses, many of which infect vertebrate hosts and are transmitted by hematophagous arthropods. Others appear to be arthropod specific, circulating only within arthropod populations. Herein, we report the isolation and characterization of three novel viruses from mosquitoes collected from the Americas. Coot Bay virus was isolated from Anopheles quadrimaculatus mosquitoes collected in the Everglades National Park, Florida; Rio Chico virus was isolated from Anopheles triannulatus mosquitoes collected in Panama; and Balsa virus was isolated from two pools of Culex erraticus mosquitoes collected in Colombia. Sequence analysis indicated that the viruses share a similar genome organization to Arboretum virus and Puerto Almendras virus that had previously been isolated from mosquitoes collected in Peru. Each genome features the five canonical rhabdovirus structural protein genes as well as a gene encoding a class 1A viroporin-like protein (U1) located between the G and L genes (3'-N-P-M-G-U1-L-5'). Phylogenetic analysis of complete L protein sequences indicated that all five viruses cluster in a unique clade that is relatively deeply rooted in the ancestry of animal rhabdoviruses. The failure of all viruses in this clade to grow in newborn mice or vertebrate cells in culture suggests that they may be poorly adapted to replication in vertebrates.

PMID:
27799634
PMCID:
PMC5239673
[Available on 2018-01-11]
DOI:
10.4269/ajtmh.16-0403
[Indexed for MEDLINE]
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