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Front Physiol. 2016 Sep 12;7:398. eCollection 2016.

Goggatomy: A Method for Opening Small Cuticular Compartments in Arthropods for Physiological Experiments.

Author information

  • 1Department of Biology, University of Iowa Iowa, IA, USA.
  • 2Department of Cellular and Molecular Physiology, Yale University New Haven, CT, USA.
  • 3Department of Chemical Engineering, University of Iowa Iowa, IA, USA.
  • 4Department of Dentistry, University of Iowa Iowa, IA, USA.

Abstract

Most sense organs of arthropods are ensconced in small exoskeletal compartments that hinder direct access to plasma membranes. We have developed a method for exposing live sensory and supporting cells in such structures. The technique uses a viscous light cured resin to embed and support the structure, which is then sliced with a sharp blade. We term the procedure a "goggatomy," from the Khoisan word for a bug, gogga. To demonstrate the utility of the method we show that it can be used to expose the auditory chordotonal organs in the second antennal segment and the olfactory receptor neurons in the third antennal segment of Drosophila melanogaster, preserving the transduction machinery. The procedure can also be used on other small arthropods, like mosquitoes and mites to expose a variety of cells.

KEYWORDS:

Drosophila melanogaster; ants; genetically encoded sensors; imaging; insects; mites; mosquitoes; sensory receptor cells

PMID:
27695420
PMCID:
PMC5025716
DOI:
10.3389/fphys.2016.00398
[PubMed - in process]
Free PMC Article
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