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Methods Mol Biol. 2016 Sep 15. [Epub ahead of print]

Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells.

Author information

1
Departments of Anesthesia and Biomedical Engineering, Yale University, New Haven, CT, 06520, USA. mahboobe.ghaedi@yale.edu.
2
Department of Biomedical Engineering, Yale University, New Haven, CT, 06520, USA. mahboobe.ghaedi@yale.edu.
3
Departments of Anesthesia and Biomedical Engineering, Yale University, New Haven, CT, 06520, USA.
4
Department of Biomedical Engineering, Yale University, New Haven, CT, 06520, USA.

Abstract

Induced pluripotent stem (iPS) cells are the product of adult somatic cell reprogramming to an embryonic-like state by inducing a "forced" expression of specific genes. They are similar to natural pluripotent stem cells, such as embryonic stem (ES) cells, in many aspects, such as the expression of certain stem cell genes and potency and differentiability. Human iPS cells are invaluable resource for basic research, cell therapy, drug discovery, and human organ tissue engineering. iPS cells can be derived from the patient to be treated and thus are genetically identical cells that may avoid immune rejection. The following protocols offer a general guideline for the induction of iPSCs from fibroblasts, and for culture and expansion to produce lung precursor cells.

KEYWORDS:

Cell culture; Differentiation; Expansion; Human induced pluripotent stem cell; Progenitors; Reprogramming

PMID:
27628133
DOI:
10.1007/7651_2016_11
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