Format

Send to

Choose Destination
Cell Rep. 2016 Aug 30;16(9):2472-85. doi: 10.1016/j.celrep.2016.07.076. Epub 2016 Aug 18.

Differential Intrasplenic Migration of Dendritic Cell Subsets Tailors Adaptive Immunity.

Author information

1
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA; Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA; Roche Pharma Research and Early Development, Roche Innovation Center Zurich, 8952 Schlieren, Switzerland.
2
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA; Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.
3
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA; Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP 14049-900, Brazil.
4
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA; Department of Neurology, The First Affiliated Hospital of Sun Yat-Sen University, Yuexiu, Guangzhou, Guangdong 510080, China.
5
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA; Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA; Respiratory, Inflammation and Autoimmunity, Innovative Medicines and Early Development, AstraZeneca, 431 50 Mölndal, Sweden.
6
The Jackson Laboratory for Genomic Medicine, Farmington, CT 06032, USA; Department of Genetics and Genome Sciences, University of Connecticut Health Center, Farmington, CT 06032, USA. Electronic address: adam.williams@jax.org.
7
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA; Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA. Electronic address: stephanie.eisenbarth@yale.edu.

Abstract

Evidence suggests that distinct splenic dendritic cell (DC) subsets activate either CD4+ or CD8+ T cells in vivo. This bias has been partially ascribed to differential antigen presentation; however, all DC subsets can activate both T cell lineages in vitro. Therefore, we tested whether the organization of DC and T cell subsets in the spleen dictated this preference. We discovered that CD4+ and CD8+ T cells segregated within splenic T cell zones prior to immunization. After intravenous immunization, the two major conventional DC populations, distinguished by 33D1 and XCR1 staining, migrated into separate regions of the T cell zone: 33D1+ DCs migrated into the CD4+ T cell area, whereas XCR1+ DCs migrated into the CD8+ T cell area. Thus, the post-immunization location of each DC subset correlated with the T cell lineage it preferentially primes. Preventing this co-localization selectively impaired either CD4+ or CD8+ T cell immunity to blood-borne antigens.

KEYWORDS:

CCR7; T cell; dendritic cell; red blood cell alloimmunization; spleen

PMID:
27545885
PMCID:
PMC6323650
DOI:
10.1016/j.celrep.2016.07.076
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center