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Mol Cell Endocrinol. 2016 Nov 15;436:169-82. doi: 10.1016/j.mce.2016.07.023. Epub 2016 Jul 21.

Mammary cell-activating factor regulates the hormone-independent transcription of the early lactation protein (ELP) gene in a marsupial.

Author information

1
School of BioSciences, The University of Melbourne, Melbourne, VIC, 3010, Australia; Cooperative Research Centre for Innovative Dairy Products, Australia. Electronic address: e.pharo@unimelb.edu.au.
2
School of BioSciences, The University of Melbourne, Melbourne, VIC, 3010, Australia. Electronic address: m.renfree@unimelb.edu.au.
3
School of BioSciences, The University of Melbourne, Melbourne, VIC, 3010, Australia; Cooperative Research Centre for Innovative Dairy Products, Australia. Electronic address: kylie.cane@gmail.com.

Abstract

The regulation of the tammar wallaby (Macropus eugenii) early lactation protein (ELP) gene is complex. ELP is responsive to the lactogenic hormones; insulin (I), hydrocortisone (HC) and prolactin (PRL) in mammary gland explants but could not be induced with lactogenic hormones in tammar primary mammary gland cells, nor in KIM-2 conditionally immortalised murine mammary epithelial cells. Similarly, ELP promoter constructs transiently-transfected into human embryonic kidney (HEK293T) cells constitutively expressing the prolactin receptor (PRLR) and Signal Transducer and Activator of Transcription (STAT)5A were unresponsive to prolactin, unlike the rat and mouse β-casein (CSN2) promoter constructs. Identification of the minimal promoter required for the hormone-independent transcription of tammar ELP in HEK293Ts and comparative analysis of the proximal promoters of marsupial ELP and the orthologous eutherian colostrum trypsin inhibitor (CTI) gene suggests that mammary cell-activating factor (MAF), an E26 transformation-specific (ETS) factor, may bind to an AGGAAG motif and activate tammar ELP.

KEYWORDS:

C/EBP; Colostrum trypsin inhibitor; ETS; Kunitz; MAF; Mammary gland

PMID:
27452799
DOI:
10.1016/j.mce.2016.07.023
[Indexed for MEDLINE]

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