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Sci Rep. 2016 Jun 30;6:28941. doi: 10.1038/srep28941.

An ABA-increased interaction of the PYL6 ABA receptor with MYC2 Transcription Factor: A putative link of ABA and JA signaling.

Author information

1
Division of Biological Sciences, Cell and Developmental Biology Section, University of California San Diego, La Jolla, California, USA.
2
Plant Biology Laboratory, Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, California, 92037 USA.
3
Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
4
Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Nagoya 464-8602, Japan.
5
Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, California, 92037 USA.

Abstract

Abscisic acid (ABA) is a plant hormone that mediates abiotic stress tolerance and regulates growth and development. ABA binds to members of the PYL/RCAR ABA receptor family that initiate signal transduction inhibiting type 2C protein phosphatases. Although crosstalk between ABA and the hormone Jasmonic Acid (JA) has been shown, the molecular entities that mediate this interaction have yet to be fully elucidated. We report a link between ABA and JA signaling through a direct interaction of the ABA receptor PYL6 (RCAR9) with the basic helix-loop-helix transcription factor MYC2. PYL6 and MYC2 interact in yeast two hybrid assays and the interaction is enhanced in the presence of ABA. PYL6 and MYC2 interact in planta based on bimolecular fluorescence complementation and co-immunoprecipitation of the proteins. Furthermore, PYL6 was able to modify transcription driven by MYC2 using JAZ6 and JAZ8 DNA promoter elements in yeast one hybrid assays. Finally, pyl6 T-DNA mutant plants show an increased sensitivity to the addition of JA along with ABA in cotyledon expansion experiments. Overall, the present study identifies a direct mechanism for transcriptional modulation mediated by an ABA receptor different from the core ABA signaling pathway, and a putative mechanistic link connecting ABA and JA signaling pathways.

PMID:
27357749
PMCID:
PMC4928087
DOI:
10.1038/srep28941
[Indexed for MEDLINE]
Free PMC Article

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