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Neurosci Lett. 2016 Aug 26;629:15-18. doi: 10.1016/j.neulet.2016.06.037. Epub 2016 Jun 21.

Tsc1 haploinsufficiency is sufficient to increase dendritic patterning and Filamin A levels.

Author information

1
Department of Neurosurgery, Xiangya Hospital, Central South University, 85 Xiangya Street, Changsha, 410008, China; Departments of Neurosurgery, and Cellular & Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520-8082, USA.
2
Departments of Neurosurgery, and Cellular & Molecular Physiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06520-8082, USA. Electronic address: angelique.bordey@yale.edu.

Abstract

Most individuals with tuberous sclerosis complex (TSC) are born with a mutant allele of either TSC1 or TSC2 and a mosaic of psychological and cognitive defects. Tsc1 loss of heterozygosity contributes to severe dendritic abnormalities that are rescued by normalizing the levels of the actin-cross linking protein, Filamin A (FLNA). However, it is unclear whether dendrites and FLNA levels are abnormal in an heterozygote Tsc1 condition. Here, we examined dendritic morphology and FLNA levels in the olfactory bulb of Tsc1 wild type and heterozygote mice. Using in vivo neonatal electroporation to label newborn neurons followed by sholl analysis, we found that Tsc1 haploinsufficiency is associated with increased dendritic complexity and total dendritic length as well as increased FLNA levels. Since reducing FLNA levels has been shown to decrease Tsc1(+/-) dendritic complexity, these data suggest that increased FLNA levels in Tsc1(+/-) mice contribute to abnormal dendritic patterning in the Tsc1 heterozygote condition of individuals with TSC.

KEYWORDS:

Circuit; Dendrite; Filamin A; Network; Neurogenesis; Tuberous sclerosis complex; mTOR

PMID:
27345385
PMCID:
PMC4983256
DOI:
10.1016/j.neulet.2016.06.037
[Indexed for MEDLINE]
Free PMC Article

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