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Cell Rep. 2016 Jun 21;15(12):2784-95. doi: 10.1016/j.celrep.2016.05.045. Epub 2016 Jun 9.

Synaptic Ribbons Require Ribeye for Electron Density, Proper Synaptic Localization, and Recruitment of Calcium Channels.

Author information

1
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520-8066, USA.
2
The Whitney Laboratory for Marine Bioscience and Department of Biology, University of Florida, St. Augustine, FL 32080, USA.
3
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520-8066, USA; Department of Surgery (Otolaryngology), Yale University School of Medicine, New Haven, CT 06520-8066, USA; Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06520-8066, USA.
4
Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, Boston, MA 02114, USA; Department of Otolaryngology, Harvard Medical School, Boston, MA 02114, USA.
5
Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520-8066, USA; Department of Opthalmology and Visual Sciences, Yale University School of Medicine, New Haven, CT 06520-8066, USA. Electronic address: david.zenisek@yale.edu.

Abstract

Synaptic ribbons are structures made largely of the protein Ribeye that hold synaptic vesicles near release sites in non-spiking cells in some sensory systems. Here, we introduce frameshift mutations in the two zebrafish genes encoding for Ribeye and thus remove Ribeye protein from neuromast hair cells. Despite Ribeye depletion, vesicles collect around ribbon-like structures that lack electron density, which we term "ghost ribbons." Ghost ribbons are smaller in size but possess a similar number of smaller vesicles and are poorly localized to synapses and calcium channels. These hair cells exhibit enhanced exocytosis, as measured by capacitance, and recordings from afferent neurons post-synaptic to hair cells show no significant difference in spike rates. Our results suggest that Ribeye makes up most of the synaptic ribbon density in neuromast hair cells and is necessary for proper localization of calcium channels and synaptic ribbons.

KEYWORDS:

exocytosis; hair cell; hearing; retina; synaptic ribbon; vestibular system

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