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Mol Biol Cell. 2016 Aug 1;27(15):2394-403. doi: 10.1091/mbc.E16-03-0165. Epub 2016 Jun 1.

Tetrahymena Poc1 ensures proper intertriplet microtubule linkages to maintain basal body integrity.

Author information

1
Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309.
2
Department of Cell and Developmental Biology, University of Colorado-Anschutz Medical Campus, Aurora, CO 80045.
3
Department of Cell and Developmental Biology, University of Colorado-Anschutz Medical Campus, Aurora, CO 80045 Chad.Pearson@ucdenver.edu Mark.Winey@colorado.edu.
4
Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, CO 80309 Chad.Pearson@ucdenver.edu Mark.Winey@colorado.edu.

Abstract

Basal bodies comprise nine symmetric triplet microtubules that anchor forces produced by the asymmetric beat pattern of motile cilia. The ciliopathy protein Poc1 stabilizes basal bodies through an unknown mechanism. In poc1∆ cells, electron tomography reveals subtle defects in the organization of intertriplet linkers (A-C linkers) that connect adjacent triplet microtubules. Complete triplet microtubules are lost preferentially near the posterior face of the basal body. Basal bodies that are missing triplets likely remain competent to assemble new basal bodies with nine triplet microtubules, suggesting that the mother basal body microtubule structure does not template the daughter. Our data indicate that Poc1 stabilizes basal body triplet microtubules through linkers between neighboring triplets. Without this stabilization, specific triplet microtubules within the basal body are more susceptible to loss, probably due to force distribution within the basal body during ciliary beating. This work provides insights into how the ciliopathy protein Poc1 maintains basal body integrity.

PMID:
27251062
PMCID:
PMC4966981
DOI:
10.1091/mbc.E16-03-0165
[Indexed for MEDLINE]
Free PMC Article

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