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Science. 2016 Jun 17;352(6292):1471-4. doi: 10.1126/science.aaf7012. Epub 2016 May 26.

Spatiotemporal coordination of stem cell commitment during epidermal homeostasis.

Author information

1
Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA.
2
Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
3
Department of Genetics, Yale School of Medicine, New Haven, CT 06510, USA. Departments of Dermatology and Cell Biology, Yale Stem Cell Center, Yale Cancer Center, Yale School of Medicine, New Haven, CT 06510, USA.

Abstract

Adult tissues replace lost cells via pools of stem cells. However, the mechanisms of cell self-renewal, commitment, and functional integration into the tissue remain unsolved. Using imaging techniques in live mice, we captured the lifetime of individual cells in the ear and paw epidermis. Our data suggest that epidermal stem cells have equal potential to either divide or directly differentiate. Tracking stem cells over multiple generations reveals that cell behavior is not coordinated between generations. However, sibling cell fate and lifetimes are coupled. We did not observe regulated asymmetric cell divisions. Lastly, we demonstrated that differentiating stem cells integrate into preexisting ordered spatial units of the epidermis. This study elucidates how a tissue is maintained by both temporal and spatial coordination of stem cell behaviors.

PMID:
27229141
PMCID:
PMC4958018
DOI:
10.1126/science.aaf7012
[Indexed for MEDLINE]
Free PMC Article
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