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Biol Reprod. 2016 Jun;94(6):134. doi: 10.1095/biolreprod.115.137497. Epub 2016 May 11.

An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline.

Author information

1
Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut Winifred.mak@yale.edu.
2
Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut.
3
Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut.
4
Yale Stem Cell Center, Yale University School of Medicine, New Haven, Connecticut Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut Division of Reproductive Endocrinology and Infertility, Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut Department of Genetics, Yale University School of Medicine, New Haven, Connecticut Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai, China Haifan.lin@yale.edu.

Abstract

Pumilio/FBF (PUF) proteins are a highly conserved family of translational regulators. The Drosophila PUF protein, Pumilio, is crucial for germline establishment and fertility. In mammals, primordial folliculogenesis is a key process that establishes the initial cohort of female mammalian germ cells prior to birth, and this primordial follicle pool is a prerequisite for female reproductive competence. We sought to understand whether PUF proteins have a conserved role in mammals during primordial folliculogenesis and female reproductive competency. In mammals, two homologs of Pumilio exist: Pumilio 1 (Pum1) and Pum2. Here, we report that PUMILIO (PUM) 1 plays an important role in the establishment of the primordial follicle pool, meiosis, and female reproductive competency, whereas PUM2 does not have a detectable function in these processes. Furthermore, we show that PUM1 facilitates the transition of the late meiotic prophase I oocyte from pachytene to diplotene stage by regulating SYCP1 protein. Our study reveals an important role of translational regulation in mammalian female germ cell development.

KEYWORDS:

Pumilio; RNA binding protein; meiosis; primordial folliculogenesis; subfertility; translational regulator

PMID:
27170441
PMCID:
PMC4946805
DOI:
10.1095/biolreprod.115.137497
[Indexed for MEDLINE]
Free PMC Article
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