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Bioorg Med Chem Lett. 2016 Jun 15;26(12):2764-2767. doi: 10.1016/j.bmcl.2016.04.074. Epub 2016 Apr 26.

Irregularities in enzyme assays: The case of macrophage migration inhibitory factor.

Author information

1
Department of Chemistry, Yale University, New Haven, CT 06520-8107, United States.
2
Department of Chemistry, Yale University, New Haven, CT 06520-8107, United States. Electronic address: william.jorgensen@yale.edu.

Abstract

Inhibitors of human macrophage migration inhibitory factor (MIF) previously reported in the literature have been reexamined by synthesis, assaying for tautomerase activity, and protein crystallography. Substantial inconsistencies between prior and current assay results are noted. They appear to arise from difficulties with the tautomerase substrates, solubility issues, and especially covalent inhibition. Incubation time variation shows that 3, 4, 6, and 9 are covalent or slow-binding inhibitors. Two protein crystal structures are provided; one confirms that the twice-discovered 3 is a covalent inhibitor.

KEYWORDS:

MIF; Protein crystallography; Reproducibility; Tautomerase assay

PMID:
27156768
DOI:
10.1016/j.bmcl.2016.04.074
[Indexed for MEDLINE]

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