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EMBO Rep. 2016 Jul;17(7):999-1012. doi: 10.15252/embr.201541708. Epub 2016 May 6.

TERRA promotes telomerase-mediated telomere elongation in Schizosaccharomyces pombe.

Author information

1
Institute of Biochemistry (IBC), Eidgenössische Technische Hochschule Zürich (ETHZ), Zürich, Switzerland.
2
Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
3
Institute of Biochemistry (IBC), Eidgenössische Technische Hochschule Zürich (ETHZ), Zürich, Switzerland claus.azzalin@bc.biol.ethz.ch.

Abstract

Telomerase-mediated telomere elongation provides cell populations with the ability to proliferate indefinitely. Telomerase is capable of recognizing and extending the shortest telomeres in cells; nevertheless, how this mechanism is executed remains unclear. Here, we show that, in the fission yeast Schizosaccharomyces pombe, shortened telomeres are highly transcribed into the evolutionarily conserved long noncoding RNA TERRA A fraction of TERRA produced upon telomere shortening is polyadenylated and largely devoid of telomeric repeats, and furthermore, telomerase physically interacts with this polyadenylated TERRA in vivo We also show that experimentally enhanced transcription of a manipulated telomere promotes its association with telomerase and concomitant elongation. Our data represent the first direct evidence that TERRA stimulates telomerase recruitment and activity at chromosome ends in an organism with human-like telomeres.

KEYWORDS:

TERRA; fission yeast; telomerase; telomere length regulation; transcription

Comment in

PMID:
27154402
PMCID:
PMC4931564
DOI:
10.15252/embr.201541708
[Indexed for MEDLINE]
Free PMC Article

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