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Regen Med. 2016 Apr;11(3):245-60. doi: 10.2217/rme-2015-0045. Epub 2016 Mar 17.

Delivery of mesenchymal stem cells in biomimetic engineered scaffolds promotes healing of diabetic ulcers.

Author information

Vascular Biology & Therapeutics Program & the Department of Surgery, Yale University School of Medicine, New Haven, CT, USA.
Department of Vascular Surgery, The Second Xiangya Hospital of Central South University, Changsha, China.
UCL Institute of Orthopaedics & Musculoskeletal Sciences, UCL Division of Surgery & Interventional Sciences, University College London, London, UK.
Departments of Diagnostic Radiology & Biomedical Engineering, Yale University, New Haven, CT, USA.
Department of Surgery, VA Connecticut Healthcare Systems, West Haven, CT, USA.



We hypothesized that delivery of mesenchymal stem cells (MSCs) in a biomimetic collagen scaffold improves wound healing in a diabetic mouse model.


Rolled collagen scaffolds containing MSCs were implanted or applied topically to diabetic C57BL/6 mice with excisional wounds.


Rolled scaffolds were hypoxic, inducing MSC synthesis and secretion of VEGF. Diabetic mice with wounds treated with rolled scaffolds containing MSCs showed increased healing compared with controls. Histologic examination showed increased cellular proliferation, increased VEGF expression and capillary density, and increased numbers of macrophages, fibroblasts and smooth muscle cells. Addition of laminin to the collagen scaffold enhanced these effects.


Activated MSCs delivered in a biomimetic-collagen scaffold enhanced wound healing in a translationally relevant diabetic mouse model.


VEGF; angiogenesis; collagen I; diabetic ulcer; extracellular matrix proteins; laminin; mesenchymal stem cells; tissue engineering; tissue scaffolds

[Indexed for MEDLINE]
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Conflict of interest statement

Financial & competing interests disclosure This work was supported in part by Yale-UCL Medtech Initiative Flagship Project Vascular Engineering award (to A Dardik, U Cheema, S Homer-Vanniasinkam), NIH Grants R56-HL095498 and R01-HL-095498 (to A Dardik), a Yale Department of Surgery Ohse award (to A Dardik), as well as through the resources and use of facilities at the Veterans Affairs Connecticut Healthcare System (West Haven, CT, USA). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.

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