Format

Send to

Choose Destination
See comment in PubMed Commons below
Sci Rep. 2016 Feb 3;6:20567. doi: 10.1038/srep20567.

Transcriptional Profiles from Paired Normal Samples Offer Complementary Information on Cancer Patient Survival--Evidence from TCGA Pan-Cancer Data.

Author information

1
Program in Computational Biology and Bioinformatics, Yale University, New Haven, CT 06520, USA.
2
Department of Pathology, School of Medicine, Yale University, New Haven, CT 06520, USA.
3
Department of Biostatistics, Yale School of Public Health, New Haven, CT 06520, USA.

Abstract

Although normal tissue samples adjacent to tumors are sometimes collected from patients in cancer studies, they are often used as normal controls to identify genes differentially expressed between tumor and normal samples. However, it is in general more difficult to obtain and clearly define paired normal samples, and whether these samples should be treated as "normal" due to their close proximity to tumors. In this article, by analyzing the accrued data in The Cancer Genome Atlas (TCGA), we show the surprising results that the paired normal samples are in general more informative on patient survival than tumors. Different lines of evidence suggest that this is likely due to tumor micro-environment instead of tumor cell contamination or field cancerization effect. Pathway analyses suggest that tumor micro-environment may play an important role in cancer patient survival either by boosting the adjacent metabolism or the in situ immunization. Our results suggest the potential benefit of collecting and profiling matched normal tissues to gain more insights on disease etiology and patient progression.

PMID:
26837275
PMCID:
PMC4738355
DOI:
10.1038/srep20567
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center