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Yale J Biol Med. 1989 Mar-Apr;62(2):159-71.

In vitro amplification techniques for the detection of nucleic acids: new tools for the diagnostic laboratory.

Author information

1
Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.

Abstract

The acceptance of nucleic acid probes as diagnostic tools for the clinical laboratory has been hampered by a number of factors, including laborious techniques and limited sensitivity. The focus of this review is on the recent development of amplification techniques to enhance the signal generated by nucleic acid-based detection systems. Three general areas are discussed: (1) amplification of target sequences using the polymerase chain reaction or the transcript amplification system, (2) amplification of the probe sequences using Q beta replicase, and (3) amplification of probe-generated signals with compound or "Christmas tree" probes. The hope of these new technologies is to simplify yet improve on the sensitivity of nucleic acid-based tests to enable them to attain a more prominent place in the diagnostic repertoire of the clinical laboratory.

PMID:
2672619
PMCID:
PMC2589213
[Indexed for MEDLINE]
Free PMC Article

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