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Arterioscler Thromb Vasc Biol. 2016 Feb;36(2):370-9. doi: 10.1161/ATVBAHA.115.306942. Epub 2015 Dec 29.

The Protein Acyl Transferase ZDHHC21 Modulates α1 Adrenergic Receptor Function and Regulates Hemodynamics.

Author information

1
From the Section of Nephrology (E.P.M., L.J., J.M., H.V.) and Department of Dermatology (L.M.M.), Yale School of Medicine, New Haven, CT; and Vascular Biology and Therapeutics Program, Department of Pharmacology, Yale University School of Medicine, New Haven, CT (A.D.L., K.F.H., A.K.L., W.C.S.). ethan.marin@yale.edu.
2
From the Section of Nephrology (E.P.M., L.J., J.M., H.V.) and Department of Dermatology (L.M.M.), Yale School of Medicine, New Haven, CT; and Vascular Biology and Therapeutics Program, Department of Pharmacology, Yale University School of Medicine, New Haven, CT (A.D.L., K.F.H., A.K.L., W.C.S.).

Abstract

OBJECTIVE:

Palmitoylation, the reversible addition of the lipid palmitate to a cysteine, can alter protein localization, stability, and function. The ZDHHC family of protein acyl transferases catalyzes palmitoylation of numerous proteins. The role of ZDHHC enzymes in intact tissue and in vivo is largely unknown. Herein, we characterize vascular functions in a mouse that expresses a nonfunctional ZDHHC21 (F233Δ).

APPROACH AND RESULTS:

Physiological studies of isolated aortae and mesenteric arteries from F233Δ mice revealed an unexpected defect in responsiveness to phenylephrine, an α1 adrenergic receptor agonist. In vivo, F233Δ mice displayed a blunted response to infusion of phenylephrine, and they were found to have elevated catecholamine levels and elevated vascular α1 adrenergic receptor gene expression. Telemetry studies showed that the F233Δ mice were tachycardic and hypotensive at baseline, consistent with diminished vascular tone. In biochemical studies, ZDHHC21 was shown to palmitoylate the α1D adrenoceptor and to interact with it in a molecular complex, thus suggesting a possible molecular mechanism by which the receptor can be regulated by ZDHHC21.

CONCLUSIONS:

Together, the data support a model in which ZDHHC21 F233Δ diminishes the function of vascular α1 adrenergic receptors, leading to reduced vascular tone, which manifests in vivo as hypotension and tachycardia. This is to our knowledge the first demonstration of a ZDHHC isoform affecting vascular function in vivo and identifies a novel molecular mode of regulation of vascular tone and blood pressure.

KEYWORDS:

adrenergic agonists; blood pressure; catecholamines; palmitate; tachycardia

PMID:
26715683
PMCID:
PMC4984414
DOI:
10.1161/ATVBAHA.115.306942
[Indexed for MEDLINE]
Free PMC Article

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