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Microscopy (Oxf). 2016 Feb;65(1):57-67. doi: 10.1093/jmicro/dfv370. Epub 2015 Dec 24.

Principles of cryo-EM single-particle image processing.

Author information

1
Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06520, USA Department of Molecular Biophysics and Biochemistry, Yale University, 333 Cedar Street, New Haven, CT 06520, USA fred.sigworth@yale.edu.

Abstract

Single-particle reconstruction is the process by which 3D density maps are obtained from a set of low-dose cryo-EM images of individual macromolecules. This review considers the fundamental principles of this process and the steps in the overall workflow for single-particle image processing. Also considered are the limits that image signal-to-noise ratio places on resolution and the distinguishing of heterogeneous particle populations.

KEYWORDS:

3D reconstruction; SNR; noise

PMID:
26705325
PMCID:
PMC4749045
DOI:
10.1093/jmicro/dfv370
[Indexed for MEDLINE]
Free PMC Article
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