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Dev Biol. 2016 Jan 15;409(2):442-50. doi: 10.1016/j.ydbio.2015.11.016. Epub 2015 Nov 30.

miR-430 regulates oriented cell division during neural tube development in zebrafish.

Author information

1
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, United States. Electronic address: carter.takacs@yale.edu.
2
Department of Genetics, Yale University School of Medicine, New Haven, CT 06510, United States; Yale Stem Cell Center, Yale University School of Medicine, New Haven, CT 06520, United States. Electronic address: antonio.giraldez@yale.edu.

Abstract

MicroRNAs have emerged as critical regulators of gene expression. Originally shown to regulate developmental timing, microRNAs have since been implicated in a wide range of cellular functions including cell identity, migration and signaling. miRNA-430, the earliest expressed microRNA during zebrafish embryogenesis, is required to undergo morphogenesis and has previously been shown to regulate maternal mRNA clearance, Nodal signaling, and germ cell migration. The functions of miR-430 in brain morphogenesis, however, remain unclear. Herein we find that miR-430 instructs oriented cell divisions in the neural rod required for neural midline formation. Loss of miR-430 function results in mitotic spindle misorientation in the neural rod, failed neuroepithelial integration after cell division, and ectopic cell accumulation in the dorsal neural tube. We propose that miR-430, independently of canonical apicobasal and planar cell polarity (PCP) pathways, coordinates the stereotypical cell divisions that instruct neural tube morphogenesis.

KEYWORDS:

MicroRNAs; Neural tube; Zebrafish

PMID:
26658217
DOI:
10.1016/j.ydbio.2015.11.016
[Indexed for MEDLINE]
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