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Cell Metab. 2016 Feb 9;23(2):324-34. doi: 10.1016/j.cmet.2015.10.017. Epub 2015 Nov 24.

Gut Commensal E. coli Proteins Activate Host Satiety Pathways following Nutrient-Induced Bacterial Growth.

Author information

1
Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
2
Microbiology Laboratory GRAM, EA2656, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
3
Microbiology Laboratory GRAM, EA2656, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France; Rouen University Hospital, CHU Charles Nicolle, Rouen 76183, France.
4
PISSARO Proteomic Platform, Mont-Saint-Aignan 76821, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
5
Animal Behavior Platform (SCAC), Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France.
6
Centre for Taste and Feeding Behaviour, UMR 6265-CNRS, 1324-INRA, Bourgogne Franche Comté University, Dijon F 21000, France.
7
Neuropharmacology Research Group, School of Pharmacy and Biomedical Sciences University of Portsmouth, Portsmouth PO 1 2DT, UK.
8
Department of Neuroscience, Karolinska Institutet, Stockholm 17176, Sweden.
9
Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France; Rouen University Hospital, CHU Charles Nicolle, Rouen 76183, France.
10
Inserm UMR1073, Nutrition, Gut and Brain Laboratory, Rouen 76183, France; Institute for Research and Innovation in Biomedicine (IRIB), Rouen University, Normandy University, Rouen 76000, France. Electronic address: serguei.fetissov@univ-rouen.fr.

Abstract

The composition of gut microbiota has been associated with host metabolic phenotypes, but it is not known if gut bacteria may influence host appetite. Here we show that regular nutrient provision stabilizes exponential growth of E. coli, with the stationary phase occurring 20 min after nutrient supply accompanied by bacterial proteome changes, suggesting involvement of bacterial proteins in host satiety. Indeed, intestinal infusions of E. coli stationary phase proteins increased plasma PYY and their intraperitoneal injections suppressed acutely food intake and activated c-Fos in hypothalamic POMC neurons, while their repeated administrations reduced meal size. ClpB, a bacterial protein mimetic of α-MSH, was upregulated in the E. coli stationary phase, was detected in plasma proportional to ClpB DNA in feces, and stimulated firing rate of hypothalamic POMC neurons. Thus, these data show that bacterial proteins produced after nutrient-induced E. coli growth may signal meal termination. Furthermore, continuous exposure to E. coli proteins may influence long-term meal pattern.

PMID:
26621107
DOI:
10.1016/j.cmet.2015.10.017
[Indexed for MEDLINE]
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