Format

Send to

Choose Destination
Cancer Biomark. 2015;15(3):299-310. doi: 10.3233/CBM-150465.

Circulating microRNA expression profile in B-cell acute lymphoblastic leukemia.

Author information

1
Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey NL, México.
2
Laboratorio de Medicina Molecular, Unidad Académica de Medicina Humana y Ciencias de la Salud, Universidad Autónoma de Zacatecas, Zacatecas, México.
3
Departamento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Monterrey NL, México.
4
Departamento de Ciencias Computacionales, ITESM, Campus Monterrey, Monterrey NL, México.
5
Centro de Investigación y Desarrollo en Ciencias de la Salud, Universidad Autónoma de Nuevo León, Monterrey, México.
6
Servicio de Hematología, Hospital Universitario Universidad Autónoma de Nuevo León, Monterrey NL, México.
7
Instituto Nacional de Medicina Genómica, México D.F. Periférico Sur, México.

Abstract

BACKGROUND:

Acute lymphoblastic leukemia (ALL) is a highly diverse disease characterized by cytogenetic and molecular abnormalities, including altered microRNA (miRNA) expression signatures.

AIM:

We perform and validate a plasma miRNA expression profiling to identify potential miRNA involved in leukemogenesis

METHODS:

MiRNA expression profiling assay was realized in 39 B-ALL and 7 normal control plasma samples using TaqMan Low Density Array (TLDA) plates on Applied Biosystems 7900 HT Fast Real-Time PCR System. MiRNA validation was done for six miRNA differentially expressed by quantitative real-time PCR.

RESULTS:

Seventy-seven circulating miRNA differentially expressed: hsa-miR-511, -222, and -34a were overexpressed, whereas hsa-miR-199a-3p, -223, -221, and -26a were underexpressed (p values < 0.005 for both sets). According to operating characteristic curve analysis, hsa-miR-511 was the most valuable biomarker for distinguishing B-ALL from normal controls, with an area under curve value of 1 and 100% for sensitivity, and specificity respectively.

CONCLUSIONS:

Measuring circulating levels of specific miRNA implicated in regulation of cell differentiation and/or cell proliferation such as hsa-miRNA-511, offers high sensitivity and specificity in B-ALL detection and may be potentially useful for detection of disease progression, as indicator of therapeutic response, and in the assessment of biological and/or therapeutic targets for patients with B-ALL.

KEYWORDS:

Biomarkers; hematological malignancies; qRT-PCR; signaling pathway

PMID:
26406572
DOI:
10.3233/CBM-150465
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for IOS Press
Loading ...
Support Center