Format

Send to

Choose Destination
Sci Rep. 2015 Aug 21;5:13378. doi: 10.1038/srep13378.

Imaging Fibrosis and Separating Collagens using Second Harmonic Generation and Phasor Approach to Fluorescence Lifetime Imaging.

Author information

1
Laboratory for Fluorescence Dynamics, Department of Biomedical Engineering, University of California Irvine, California.
2
Gene Expression Laboratory, Salk Institute for Biological Studies, La Jolla, CA.
3
Division of Renal Diseases and Hypertension, University of Colorado at Denver, Aurora, Colorado.

Abstract

In this paper we have used second harmonic generation (SHG) and phasor approach to auto fluorescence lifetime imaging (FLIM) to obtain fingerprints of different collagens and then used these fingerprints to observe bone marrow fibrosis in the mouse femur. This is a label free approach towards fast automatable detection of fibrosis in tissue samples. FLIM has previously been used as a method of contrast in different tissues and in this paper phasor approach to FLIM is used to separate collagen I from collagen III, the markers of fibrosis, the largest groups of disorders that are often without any effective therapy. Often characterized by an increase in collagen content of the corresponding tissue, the samples are usually visualized by histochemical staining, which is pathologist dependent and cannot be automated.

PMID:
26293987
PMCID:
PMC4543938
DOI:
10.1038/srep13378
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center