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Cytoskeleton (Hoboken). 2015 Aug;72(8):373-87. doi: 10.1002/cm.21236.

Myosin VI and cardiomyopathy: Left ventricular hypertrophy, fibrosis, and both cardiac and pulmonary vascular endothelial cell defects in the Snell's waltzer mouse.

Author information

1
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut.
2
Department of Internal Medicine, Yale School of Medicine, New Haven, Connecticut.
3
Department of Cell Biology, Yale School of Medicine, New Haven, Connecticut.
4
Department of Pathology, Yale School of Medicine, New Haven, Connecticut.

Abstract

In mice and humans, loss of myosin VI (Myo6) function results in deafness, and certain Myo6 mutations also result in cardiomyopathies in humans. The current studies have utilized the Snell's waltzer (sv) mouse (a functional null mutation for Myo6) to determine if this mouse also exhibits cardiac defects and thus used to determine the cellular and molecular basis for Myo6-associated heart disease. Myo6 is expressed in mouse heart where it is predominantly expressed in vascular endothelial cells (VECs) based on co-localization with the VEC cell marker CD31. Sv/sv heart mass is significantly greater than that of sv/+ littermates, a result of left ventricle hypertrophy. The left ventricle of the sv/sv exhibits extensive fibrosis, both interstitial and perivascular, based on histologic staining, and immunolocalization of several markers for fibrosis including fibronectin, collagen IV, and the fibroblast marker vimentin. Myo6 is also expressed in lung VECs but not in VECs of intestine, kidney, or liver. Sv/sv lungs exhibit increased periaveolar fibrosis and enlarged air sacs. Electron microscopy of sv/sv cardiac and lung VECs revealed abnormal ultrastructure, including luminal protrusions and increased numbers of cytoplasmic vesicles. Previous studies have shown that loss of function of either Myo6 or its adaptor binding partner synectin/GIPC results in impaired arterial development due to defects in VEGF signaling. However, examination of synectin/GIPC-/- heart revealed no fibrosis or significantly altered VEC ultrastructure, suggesting that the cardiac and lung defects observed in the sv/sv mouse are not due to Myo6 function in arterial development.

KEYWORDS:

GIPC; Myo6; caveoli; fibrosis; vascular endothelial cell

PMID:
26265212
PMCID:
PMC4600664
DOI:
10.1002/cm.21236
[Indexed for MEDLINE]
Free PMC Article

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