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Methods Cell Biol. 2015;129:301-315. doi: 10.1016/bs.mcb.2015.03.003. Epub 2015 May 27.

Time-lapse recording of centrosomes and other organelles in Drosophila neuroblasts.

Author information

1
Institute for Research in Biomedicine (IRB-Barcelona), Barcelona, Spain.
2
Division of Cell and Developmental Biology, College of Life Sciences, University of Dundee, Dundee, UK.
3
Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal; IBMC-Instituto de Biologia Molecular e Celular, Universidade do Porto, Portugal.
4
Institute for Research in Biomedicine (IRB-Barcelona), Barcelona, Spain; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain.

Abstract

Drosophila larval neuroblasts (NBs) are an excellent model for asymmetric division and cell cycle studies in general. For decades, visualizing relevant structures like centrosomes, chromosomes, or the mitotic spindle relied exclusively on immunofluorescence on fix samples. More recently, improvements on sensitivity and acquisition speed of different confocal systems have made it possible to acquire time-resolved images of combined fluorescent reporters from single larval NBs. Here, we provide protocols to visualize centrosomes and other organelles from both primary cultures of isolated single NBs and ex vivo, whole-mounted larval brains.

KEYWORDS:

Cell culture; Centriole; Centrosome; Drosophila; Microscopy; Neuroblast

PMID:
26175445
DOI:
10.1016/bs.mcb.2015.03.003
[Indexed for MEDLINE]

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