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Carbohydr Res. 2015 Sep 2;413:115-22. doi: 10.1016/j.carres.2015.05.015. Epub 2015 Jun 9.

Structure determination of the neutral exopolysaccharide produced by Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1.

Author information

1
Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Boulevard West, Saint-Hyacinthe, Québec J2S 8E3, Canada. Electronic address: Marie-Rose.VanCalsteren@AGR.GC.CA.
2
Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Boulevard West, Saint-Hyacinthe, Québec J2S 8E3, Canada.
3
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aobaku, Sendai 981-8555, Japan.
4
Lactic Acid Bacteria Research Department, Food Science Research Laboratories, Meiji Co., Ltd., 540 Naruda, Odawara, Kanagawa 250-0862, Japan.

Abstract

The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp. bulgaricus strain OLL1073R-1 was purified and characterized. The molecular mass was 5.0×10(6) g/mol. Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5. The NPS was also submitted to periodate oxidation followed by borohydride reduction and Smith degradation. Sugar and methylation analyses, (1)H and (13)C nuclear magnetic resonance, and mass spectrometry of the NPS or of its specifically modified products allowed determining the repeating unit sequence: {2)Glc(α1-3)Glc(β1-3)[Gal(β1-4)]Gal(β1-4)Gal(α1-}n. The structure is compared to that of exopolysaccharides produced by other Lactobacillus bulgaricus strains.

KEYWORDS:

Mass spectrometry (MS); Nuclear magnetic resonance (NMR); Repeating unit sequence; Smith degradation; casper

PMID:
26117825
DOI:
10.1016/j.carres.2015.05.015
[Indexed for MEDLINE]

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