Send to

Choose Destination
See comment in PubMed Commons below
Biomark Insights. 2015 Jun 10;10(Suppl 1):91-103. doi: 10.4137/BMI.S23912. eCollection 2015.

Prospects for In Vitro Myofilament Maturation in Stem Cell-Derived Cardiac Myocytes.

Author information

Department of Biomedical Engineering, Yale University, New Haven, CT, USA.


Cardiomyocytes derived from human stem cells are quickly becoming mainstays of cardiac regenerative medicine, in vitro disease modeling, and drug screening. Their suitability for such roles may seem obvious, but assessments of their contractile behavior suggest that they have not achieved a completely mature cardiac muscle phenotype. This could be explained in part by an incomplete transition from fetal to adult myofilament protein isoform expression. In this commentary, we review evidence that supports this hypothesis and discuss prospects for ultimately generating engineered heart tissue specimens that behave similarly to adult human myocardium. We suggest approaches to better characterize myofilament maturation level in these in vitro systems, and illustrate how new computational models could be used to better understand complex relationships between muscle contraction, myofilament protein isoform expression, and maturation.


cardiomyopathy; disease modeling; engineered heart tissue human pluripotent stem cell-derived cardiomyocytes; maturation; protein isoforms

PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Libertas Academica Icon for PubMed Central
    Loading ...
    Support Center