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Am J Clin Pathol. 2015 May;143(5):694-700. doi: 10.1309/AJCPCZA4PSZCFHQ4.

Detection and genotype of high-risk human papillomavirus in fine-needle aspirates of patients with metastatic squamous cell carcinoma is helpful in determining tumor origin.

Author information

1
From the Department of Pathology, Yale University School of Medicine, New Haven, CT.
2
From the Department of Pathology, Yale University School of Medicine, New Haven, CT. David.Chhieng@yale.edu.

Abstract

OBJECTIVES:

Recent studies have shown that human papillomavirus (HPV) is associated with a certain subset of head and neck squamous cell carcinoma (HNSCC)-namely, those arising in the oropharynx. The objective of this study is to determine the efficacy, detection, and genotype of high-risk (HR) HPV using the Roche cobas 4800 system (Roche Molecular System, Pleasanton, CA).

METHODS:

Forty-two fine-needle aspirate (FNA) specimens from 37 patients with cervical (n = 36) or mediastinal (n = 5) lymphadenopathy or a left parapharyngeal mass (n =1) were included in this prospective study. HR-HPV testing was performed on residual FNA material after direct smear preparation and, if positive, was further delineated into HPV 16/18 genotypes using the Roche cobas 4800 system. Follow-up included review of histologic material and/or electronic health records.

RESULTS:

Among those HNSCCs that were positive for HR-HPV, 18 (100%) of 18 originated from the oropharynx, whereas only two (13%) of 15 HR-HPV-negative HNSCCs originated from the oropharynx (χ(2) test, P < .05). p16 immunohistochemical assay and HPV 16 in situ hybridization on corresponding histologic specimens were concordant with cytologic HR-HPV results.

CONCLUSIONS:

HR-HPV detection and genotyping can be performed on lymph node FNAs with metastatic squamous cell carcinoma using the Roche cobas 4800 system. The presence of HR-HPV and/or HPV 16 is a reliable indicator of the metastatic squamous cell carcinoma originating from the oropharynx.

KEYWORDS:

Cytology; Cytopathology; Head/neck; Molecular diagnostics

PMID:
25873503
DOI:
10.1309/AJCPCZA4PSZCFHQ4
[Indexed for MEDLINE]

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