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Int J Mol Sci. 2015 Apr 2;16(4):7462-77. doi: 10.3390/ijms16047462.

The neuronal-specific SGK1.1 (SGK1_v2) kinase as a transcriptional modulator of BAG4, Brox, and PPP1CB genes expression.

Author information

  • 1Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, Av Astrofísico Sánchez s/n, La Laguna, Tenerife 38201, Spain. refernan@ull.es.
  • 2Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, Av Astrofísico Sánchez s/n, La Laguna, Tenerife 38201, Spain. javila@ull.es.
  • 3Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06510, USA. marifrancis.arteaga@gmail.com.
  • 4Department of Medicine A, Hematology, Hemostaseology, Oncology and Pneumology, University of Muenster, Muenster 48149, Germany. marifrancis.arteaga@gmail.com.
  • 5Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06510, USA. cecilia.canessa@yale.edu.
  • 6Department of Basic Medical Sciences, Tsinghua University School of Medicine, Beijing 100084, China. cecilia.canessa@yale.edu.
  • 7Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, Av Astrofísico Sánchez s/n, La Laguna, Tenerife 38201, Spain. pmartin@ull.es.

Abstract

The Serum- and Glucocorticoid-induced Kinase 1, SGK1, exhibits a broad range of cellular functions that include regulation of the number of ion channels in plasma membrane and modulation of signaling pathways of cell survival. This diversity of functions is made possible by various regulatory processes acting upon the SGK1 gene, giving rise to various isoforms: SGK1_v1-5, each with distinct properties and distinct aminotermini that serve to target proteins to different subcellular compartments. Among cellular effects of SGK1 expression is to indirectly modulate gene transcription by phosphorylating transcriptional factors of the FOXO family. Here we examined if SGK1.1 (SGK1_v2; NM_001143676), which associates primarily to the plasma membrane, is also able to regulate gene expression. Using a differential gene expression approach we identified six genes upregulated by SGK1.1 in HeLa cells. Further analysis of transcript and protein levels validated two genes: BCL2-associated athanogene 4 (BAG-4) and Brox. The results indicate that SGK1.1 regulates gene transcription upon a different set of genes some of which participate in cell survival pathways (BAG-4) and others in intracellular vesicular traffic (Brox).

PMID:
25849655
PMCID:
PMC4425028
DOI:
10.3390/ijms16047462
[PubMed - indexed for MEDLINE]
Free PMC Article
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