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Blood. 2015 May 28;125(22):3377-87. doi: 10.1182/blood-2015-01-620278. Epub 2015 Mar 26.

Hyperglycemia repression of miR-24 coordinately upregulates endothelial cell expression and secretion of von Willebrand factor.

Author information

1
Section of Cardiovascular Medicine, Department of Internal Medicine, Yale Cardiovascular Research Center.
2
Department of Genetics, Yale Stem Cell Center.
3
Section of Endocrinology and Metabolism, Yale Diabetes Center, and.
4
Section of Pulmonary Medicine, Yale University School of Medicine, New Haven, CT.

Abstract

An elevated level of von Willebrand factor (VWF) in diabetic patients is associated with increased risk of thrombotic cardiovascular events. The underlying mechanism of how VWF expression is upregulated in diabetes mellitus is poorly understood. We now report that hyperglycemia-induced repression of microRNA-24 (miR-24) increases VWF expression and secretion in diabetes mellitus. In diabetic patients and diabetic mouse models (streptozotocin/high-fat diet-induced and db/db mice), miR-24 is reduced in both tissues and plasma. Knockdown of miR-24 in mice leads to increased VWF mRNA and protein levels and enhanced platelet tethering (spontaneous thrombosis). miR-24 tightly controls VWF levels through pleiotropic effects, including direct binding to the 3' untranslated region of VWF and targeting FURIN and the histamine H1 receptor, known regulators of VWF processing and secretion in endothelial cells. We present a novel mechanism for miR-24 downregulation through hyperglycemia-induced activation of aldose reductase, reactive oxygen species, and c-Myc. These findings support a critical role for hyperglycemic repression of miR-24 in VWF-induced pathology. miR-24 represents a novel therapeutic target to prevent adverse thrombotic events in patients with diabetes mellitus.

Comment in

PMID:
25814526
PMCID:
PMC4447857
DOI:
10.1182/blood-2015-01-620278
[Indexed for MEDLINE]
Free PMC Article
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